Title | A Biomimetic Phosphatidylcholine-Terminated Monolayer Greatly Improves the In Vivo Performance of Electrochemical Aptamer-Based Sensors. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Li H, Dauphin-Ducharme P, Arroyo-Currás N, Tran CH, Vieira PA, Li S, Shin C, Somerson J, Kippin TE, Plaxco KW |
Journal | Angew Chem Int Ed Engl |
Volume | 56 |
Issue | 26 |
Pagination | 7492-7495 |
Date Published | 2017 Jun 19 |
ISSN | 1521-3773 |
Abstract | The real-time monitoring of specific analytes in situ in the living body would greatly advance our understanding of physiology and the development of personalized medicine. Because they are continuous (wash-free and reagentless) and are able to work in complex media (e.g., undiluted serum), electrochemical aptamer-based (E-AB) sensors are promising candidates to fill this role. E-AB sensors suffer, however, from often-severe baseline drift when deployed in undiluted whole blood either in vitro or in vivo. We demonstrate that cell-membrane-mimicking phosphatidylcholine (PC)-terminated monolayers improve the performance of E-AB sensors, reducing the baseline drift from around 70 % to just a few percent after several hours in flowing whole blood in vitro. With this improvement comes the ability to deploy E-AB sensors directly in situ in the veins of live animals, achieving micromolar precision over many hours without the use of physical barriers or active drift-correction algorithms. |
DOI | 10.1002/anie.201700748 |
Alternate Journal | Angew. Chem. Int. Ed. Engl. |
PubMed ID | 28371090 |
PubMed Central ID | PMC5660315 |
Grant List | F31 CA183385 / CA / NCI NIH HHS / United States R01 AI107936 / AI / NIAID NIH HHS / United States |