A Biomimetic Phosphatidylcholine-Terminated Monolayer Greatly Improves the In Vivo Performance of Electrochemical Aptamer-Based Sensors.

TitleA Biomimetic Phosphatidylcholine-Terminated Monolayer Greatly Improves the In Vivo Performance of Electrochemical Aptamer-Based Sensors.
Publication TypeJournal Article
Year of Publication2017
AuthorsLi H, Dauphin-Ducharme P, Arroyo-Currás N, Tran CH, Vieira PA, Li S, Shin C, Somerson J, Kippin TE, Plaxco KW
JournalAngew Chem Int Ed Engl
Volume56
Issue26
Pagination7492-7495
Date Published2017 Jun 19
ISSN1521-3773
Abstract

The real-time monitoring of specific analytes in situ in the living body would greatly advance our understanding of physiology and the development of personalized medicine. Because they are continuous (wash-free and reagentless) and are able to work in complex media (e.g., undiluted serum), electrochemical aptamer-based (E-AB) sensors are promising candidates to fill this role. E-AB sensors suffer, however, from often-severe baseline drift when deployed in undiluted whole blood either in vitro or in vivo. We demonstrate that cell-membrane-mimicking phosphatidylcholine (PC)-terminated monolayers improve the performance of E-AB sensors, reducing the baseline drift from around 70 % to just a few percent after several hours in flowing whole blood in vitro. With this improvement comes the ability to deploy E-AB sensors directly in situ in the veins of live animals, achieving micromolar precision over many hours without the use of physical barriers or active drift-correction algorithms.

DOI10.1002/anie.201700748
Alternate JournalAngew. Chem. Int. Ed. Engl.
PubMed ID28371090
PubMed Central IDPMC5660315
Grant ListF31 CA183385 / CA / NCI NIH HHS / United States
R01 AI107936 / AI / NIAID NIH HHS / United States